Korelasi Ekspresi MMP-9 dengan Derajat Histologik dan Faktor Karakteristik Derajat Histologik pada Karsinoma Payudara Invasif

Abstract

Background
Histological grade and MMP-9 expression are prognostic factors in invasive breast carcinoma, nevertheless the
correlation between both prognostic factors is still debatable. The aim of this study was to determine the
relationship between expression of MMP-9 and histological grade and to evaluate which characteristic factor of
the histological grade is dominant to MMP-9 expression in invasive breast carcinoma.
Methods
This study was carried out using a cross sectional analytical method. The samples were paraffin blocks from
invasive carcinoma of no special type grade I, II, and III at Pathology Anatomy Department Udayana
University/RSUP Sanglah Denpasar from 1st January 2013 to 31st July 2016. Histopathologically re-diagnosis
was conducted to get the eligible samples and obtained 47 samples, consisted of 7 samples grade I, 18
samples grade II, and 22 samples grade III. Immunohistochemistry of the MMP-9 was done and the result was
correlated to histological grade and characteristic factor of histological grade.
Results
The study results showed high expression of MMP-9 2.1% (n=1) in grade I, 19.2% (n=9) in grade II, and 36.2%
(n=17) in grade III. Chi-square test analysis showed there was a significant correlation between tumor grade
and MMP-9 expression (p=0,010). The logistic regression test showed the mitotic counting was statistically
significant dominant factor (p=0.012) determining MMP-9 expression, whereas tubular formation and nuclear
pleomorfia were not statistically significant (p>0.05).
Conclusion
High expression of MMP-9 was correlated with high histological grade, which also indicated worse prognosis.
MMP-9 testing is an important examination to determine the level of tumour aggresive-ness and plan the
effective therapies